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annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit  (Beyotime)


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    Beyotime annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit
    Annexin V Fitc And Pi Reagents In Binding Buffer Using The Annexin V Fitc/Pi Cell Apoptosis Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer+cell+apoptosis+kit/annexin+v+fitc+and+pi+reagents+in+binding+buffer+using+the+annexin+v+fitc+pi+cell+apoptosis+detection+kit/pmc04933872-32-32-48
    Average 90 stars, based on 1 article reviews
    annexin v-fitc and pi reagents in binding buffer using the annexin v-fitc/pi cell apoptosis detection kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Sterility:

    Article Title: Protective Effects of a Jellyfish-Derived Thioredoxin Fused with Cell-Penetrating Peptide TAT-PTD on H 2 O 2 -Induced Oxidative Damage.
    Article Snippet: .. Afterward, cells were washed with ice-cold PBS, gently collected into sterile centrifuge tubes, and resuspended in 200 μL of the binding buffer from an apoptosis analysis kit (Beyotime, Shanghai, China). ..

    Binding Assay:

    Article Title: Protective Effects of a Jellyfish-Derived Thioredoxin Fused with Cell-Penetrating Peptide TAT-PTD on H 2 O 2 -Induced Oxidative Damage.
    Article Snippet: .. Afterward, cells were washed with ice-cold PBS, gently collected into sterile centrifuge tubes, and resuspended in 200 μL of the binding buffer from an apoptosis analysis kit (Beyotime, Shanghai, China). ..

    Article Title: Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract
    Article Snippet: HT-29 cells were treated with 0.2 mg/mL HSGT solution of different storage years for 24 h or 48 h, in which 5 μM cisplatin (MedChemExpress, Princeton, NJ, USA) was used as a positive control for apoptosis. .. A total of 1 × 10 5 digested HT-29 cells were resuspended with 195 μL Annexin V-FITC binding solution (C1062L Annexin V-FITC Apoptosis Detection Kit, Beyotime Biotechnology), and then 5 μL fluorescein isothiocyanate (FITC) and 10 μL PI staining solution were added. ..

    Article Title: Jianpi-Qinghua Formula alleviates liver dysfunction by suppression of HBV replication in HepG2.2.15 cells
    Article Snippet: .. After treatment, cells were harvested with 0.25% trypsin-EDTA, washed twice with cold PBS, and resuspended in 1× Binding Buffer (Annexin V-FITC/PI Apoptosis Detection Kit, Beyotime biotechnology, China). ..

    Article Title: Use of nocardia rubra cell wall skeleton in regenerative medicine
    Article Snippet: .. 500 μl of binding buffer (Annexin V-FITC apoptosis detection kit, Shanghai Beyotime Biotechnology Co., Ltd.), 5 μl of FITC and 5 μl of PI were successively added to each tube. ..

    Article Title: Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract.
    Article Snippet: HT-29 cells were treated with 0.2 mg/mL HSGT solution of different storage years for 24 h or 48 h, in which 5 μM cisplatin (MedChemExpress, Princeton, NJ, USA) was used as a positive control for apoptosis. .. A total of 1 × 105 digested HT-29 cells were resuspended with 195 μL Annexin V-FITC binding solution (C1062L Annexin V-FITC Apoptosis Detection Kit, Beyotime Biotechnology), and then 5 μL fluorescein isothiocyanate (FITC) and 10 μL PI staining solution were added. ..

    Article Title: Physicochemical and biological properties of composite bone cement based on octacalcium phosphate and tricalcium silicate
    Article Snippet: Biocompatible tricalcium silicate (C3S) bone cement is widely used as dental and bone repair material; however, its long setting time, poor injectability and low initial mechanical properties limit clinical applications.. In order to improve C3S silicate bone cement and its derivatives to play a more important role in tooth restoration, bone defect repair, implant coating and tissue engineering scaffolds, a novel C3S and octacalcium phosphate (OCP) composite bone cement (OCP/C3S) was prepared and evaluated for setting time, injectability, anti-flocculation, pH, microstructure, bioactivity and cytotoxicity.. The setting time of the OCP/C3S composite bone cement was controlled within the clinically operable time range (8.3–13.7 min); the cement exhibited good compressive strength, injectability (93.54%), and anti-collapse performance.

    Staining:

    Article Title: Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract
    Article Snippet: HT-29 cells were treated with 0.2 mg/mL HSGT solution of different storage years for 24 h or 48 h, in which 5 μM cisplatin (MedChemExpress, Princeton, NJ, USA) was used as a positive control for apoptosis. .. A total of 1 × 10 5 digested HT-29 cells were resuspended with 195 μL Annexin V-FITC binding solution (C1062L Annexin V-FITC Apoptosis Detection Kit, Beyotime Biotechnology), and then 5 μL fluorescein isothiocyanate (FITC) and 10 μL PI staining solution were added. ..

    Article Title: Induction of Apoptosis via Inactivating PI3K/AKT Pathway in Colorectal Cancer Cells Using Aged Chinese Hakka Stir-Fried Green Tea Extract.
    Article Snippet: HT-29 cells were treated with 0.2 mg/mL HSGT solution of different storage years for 24 h or 48 h, in which 5 μM cisplatin (MedChemExpress, Princeton, NJ, USA) was used as a positive control for apoptosis. .. A total of 1 × 105 digested HT-29 cells were resuspended with 195 μL Annexin V-FITC binding solution (C1062L Annexin V-FITC Apoptosis Detection Kit, Beyotime Biotechnology), and then 5 μL fluorescein isothiocyanate (FITC) and 10 μL PI staining solution were added. ..

    other:

    Article Title: Stabilized iRGD modification enhances NY-ESO-1 TCR-T infiltration in solid tumors and synergizes with PD-1 blockade
    Article Snippet: For apoptosis assessment, 10 5 cells were collected for each sample and resuspended in 100 μL of 1 × Binding Buffer (Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit, Beyotime, Cat# C1062, China).

    Article Title: Stabilized iRGD modification enhances NY-ESO-1 TCR-T infiltration in solid tumors and synergizes with PD-1 blockade.
    Article Snippet: For apoptosis assessment, 105 cells were collected for each sample and resuspended in 100 μL of 1 × Binding Buffer (Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit, Beyotime, Cat# C1062, China).

    Blocking Assay:

    Article Title: Physicochemical and biological properties of composite bone cement based on octacalcium phosphate and tricalcium silicate
    Article Snippet: Biocompatible tricalcium silicate (C3S) bone cement is widely used as dental and bone repair material; however, its long setting time, poor injectability and low initial mechanical properties limit clinical applications.. In order to improve C3S silicate bone cement and its derivatives to play a more important role in tooth restoration, bone defect repair, implant coating and tissue engineering scaffolds, a novel C3S and octacalcium phosphate (OCP) composite bone cement (OCP/C3S) was prepared and evaluated for setting time, injectability, anti-flocculation, pH, microstructure, bioactivity and cytotoxicity.. The setting time of the OCP/C3S composite bone cement was controlled within the clinically operable time range (8.3–13.7 min); the cement exhibited good compressive strength, injectability (93.54%), and anti-collapse performance.



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    Image Search Results


    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control, Standard Deviation